Fundamentals of Fluorescence Microscopy

Find basic definitions for common microscopy terms; understand magnification and how fluorescence can be used to improve contrast and increase resolution.

Fluorescence microscopy uses fluorescence and phosphorescence to examine the structural organization, spatial distribution of samples. It is particularly used ...

A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or ...

由 MJ Sanderson 著作 · 2014 · 被引用 487 次 — Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical ...

Learn the basic physics behind the excitation and emission of a fluorophore and how microscopes channel and capture the light generated to make an image.

由 JW Lichtman 著作 · 2005 · 被引用 2370 次 — Fluorescence microscopy is the quintessential example, as it aims to reveal only the objects of interest in an otherwise black background.

The basic function of a fluorescence microscope is to irradiate the specimen with a desired and specific band of wavelengths, and then to separate the much ...

2022年4月12日 — A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and ...

The key to fluorescence microscopy is the use of appropriate filters to segregate the intense excitation light from the much weaker secondary emission generated ...